## ----设置,回声=假-------------------------------------------- knitr :: Opts_chunk $ set(cache = true)## ---- deseq ------------------------------------------------------- - 要求(“deseq2”)要求(“甲状旁腺”)数据(“ParathyroidGenesse”)SE < - 甲状旁腺特异性Colnames(SE)< - SE $ RUN ## ---- DESEQ-PREP ---------------------------------------------------- DDSFULL < - DESEQDATASET(SE,Design =〜患者+治疗)DDSCollapsed < - 折叠事件(DDSFULL,GroupBy = DDSFULL $ SAMPLE,RUN = DDSFULL $ RUN)DDS < - DDSCOLLAPSED [,DDSCOLLAPSED $ TIME ==“48H”] DDS $ TIME < - DREPLEVES(DDS $ TIME)DDS $治疗< - 相关性(DDS $待遇,“控制”)## ---- DESEQ分析-------------------------------------------------------------- DDS < - DESQ(DDS)RES < - 结果(DDS,对比度= C(“处理”,“DPN”,“控制”))## ---- DESEQ-解释------------------------------------------------- - ressig < - res [哪个(res $ padj <0.1),]头(ressig [订单(ressig $ log2foldchange),])##---- deseq-ma -------------------------------------------------------------------------- plotma(res,ylim = c(-1,1))## ---- Deseq-Dispestest ----------------------------------------------------------------,DDS,Ylim = C(1E-6,1E1))##---- deseq-stay ---------------------------------------------------神父(res $ pvalue,breaks = 40,col =“灰色”)